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protoscript ii rt pcr kit  (TaKaRa)


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    TaKaRa protoscript ii rt pcr kit
    Tamoxifen ( TAM ) induced glioma cell autophagy. A, U87‐ MG cells were treated with 5 μmol/L TAM for different time periods. Mitochondrial membrane potentia was analyzed using flow cytometry. B, Western blot analysis of the expression of P53 and Bcl‐2. C, Cells were treated with 5 μmol/L TAM for different time periods, and then stained with acridine orange and examined under a fluorescence microscope. D, Western blot analysis of the accumulation of LC 3‐II in U87‐ MG cells that were treated with TAM (5 μmol/L) for different time period. E, p‐Akt, Akt, p‐ mTOR and mTOR protein levels were estimated by western blot analysis. F, RT ‐ <t>PCR</t> examination of autophagy pathway in mRNA expression. Beclin‐1, ATG ‐5, ATG ‐12, P62 (n = 5, * P < 0.05, ** P < .01 vs control). mTOR , mammalian target of rapamycin
    Protoscript Ii Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 730 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protoscript ii rt pcr kit/product/TaKaRa
    Average 98 stars, based on 730 article reviews
    protoscript ii rt pcr kit - by Bioz Stars, 2026-02
    98/100 stars

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    1) Product Images from "Estrogen receptor variant ER ‐α36 promotes tamoxifen agonist activity in glioblastoma cells"

    Article Title: Estrogen receptor variant ER ‐α36 promotes tamoxifen agonist activity in glioblastoma cells

    Journal: Cancer Science

    doi: 10.1111/cas.13868

    Tamoxifen ( TAM ) induced glioma cell autophagy. A, U87‐ MG cells were treated with 5 μmol/L TAM for different time periods. Mitochondrial membrane potentia was analyzed using flow cytometry. B, Western blot analysis of the expression of P53 and Bcl‐2. C, Cells were treated with 5 μmol/L TAM for different time periods, and then stained with acridine orange and examined under a fluorescence microscope. D, Western blot analysis of the accumulation of LC 3‐II in U87‐ MG cells that were treated with TAM (5 μmol/L) for different time period. E, p‐Akt, Akt, p‐ mTOR and mTOR protein levels were estimated by western blot analysis. F, RT ‐ PCR examination of autophagy pathway in mRNA expression. Beclin‐1, ATG ‐5, ATG ‐12, P62 (n = 5, * P < 0.05, ** P < .01 vs control). mTOR , mammalian target of rapamycin
    Figure Legend Snippet: Tamoxifen ( TAM ) induced glioma cell autophagy. A, U87‐ MG cells were treated with 5 μmol/L TAM for different time periods. Mitochondrial membrane potentia was analyzed using flow cytometry. B, Western blot analysis of the expression of P53 and Bcl‐2. C, Cells were treated with 5 μmol/L TAM for different time periods, and then stained with acridine orange and examined under a fluorescence microscope. D, Western blot analysis of the accumulation of LC 3‐II in U87‐ MG cells that were treated with TAM (5 μmol/L) for different time period. E, p‐Akt, Akt, p‐ mTOR and mTOR protein levels were estimated by western blot analysis. F, RT ‐ PCR examination of autophagy pathway in mRNA expression. Beclin‐1, ATG ‐5, ATG ‐12, P62 (n = 5, * P < 0.05, ** P < .01 vs control). mTOR , mammalian target of rapamycin

    Techniques Used: Flow Cytometry, Western Blot, Expressing, Staining, Fluorescence, Microscopy, Reverse Transcription Polymerase Chain Reaction

    Overexpression of ER ‐α36 in U251 cells leads to decreased sensitivity to tamoxifen ( TAM ) and enhanced autophagy. U251 cells overexpressed ER ‐α36. A, RT ‐ PCR examination of ER ‐α36 expression. B, MTT analysis of cell viability of glioma cells. C, Western blot analysis of the accumulation of LC 3‐II (n = 4, ** P < .01, * P < .05 vs control). ER , estrogen receptor
    Figure Legend Snippet: Overexpression of ER ‐α36 in U251 cells leads to decreased sensitivity to tamoxifen ( TAM ) and enhanced autophagy. U251 cells overexpressed ER ‐α36. A, RT ‐ PCR examination of ER ‐α36 expression. B, MTT analysis of cell viability of glioma cells. C, Western blot analysis of the accumulation of LC 3‐II (n = 4, ** P < .01, * P < .05 vs control). ER , estrogen receptor

    Techniques Used: Over Expression, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot

    U251/ TAM shows increased levels of ER ‐α36, together with increased cell autophagy. A, RT‐PCR examination of ER‐α36 expression in U251 and U251/TAM. B, Knockdown of ER‐α36 by shRNA method. RT‐PCR examination of ER‐α36 expression in U251/TAM‐V and U251/TAM‐siRNA. C, MTT analysis of cell viability of glioma cells. D, Western blot analysis of accumulated LC 3‐II (n = 4, ** P < .01 vs control). ER , estrogen receptor; TAM, tamoxifen
    Figure Legend Snippet: U251/ TAM shows increased levels of ER ‐α36, together with increased cell autophagy. A, RT‐PCR examination of ER‐α36 expression in U251 and U251/TAM. B, Knockdown of ER‐α36 by shRNA method. RT‐PCR examination of ER‐α36 expression in U251/TAM‐V and U251/TAM‐siRNA. C, MTT analysis of cell viability of glioma cells. D, Western blot analysis of accumulated LC 3‐II (n = 4, ** P < .01 vs control). ER , estrogen receptor; TAM, tamoxifen

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, shRNA, Western Blot



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    Tamoxifen ( TAM ) induced glioma cell autophagy. A, U87‐ MG cells were treated with 5 μmol/L TAM for different time periods. Mitochondrial membrane potentia was analyzed using flow cytometry. B, Western blot analysis of the expression of P53 and Bcl‐2. C, Cells were treated with 5 μmol/L TAM for different time periods, and then stained with acridine orange and examined under a fluorescence microscope. D, Western blot analysis of the accumulation of LC 3‐II in U87‐ MG cells that were treated with TAM (5 μmol/L) for different time period. E, p‐Akt, Akt, p‐ mTOR and mTOR protein levels were estimated by western blot analysis. F, RT ‐ <t>PCR</t> examination of autophagy pathway in mRNA expression. Beclin‐1, ATG ‐5, ATG ‐12, P62 (n = 5, * P < 0.05, ** P < .01 vs control). mTOR , mammalian target of rapamycin
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    Tamoxifen ( TAM ) induced glioma cell autophagy. A, U87‐ MG cells were treated with 5 μmol/L TAM for different time periods. Mitochondrial membrane potentia was analyzed using flow cytometry. B, Western blot analysis of the expression of P53 and Bcl‐2. C, Cells were treated with 5 μmol/L TAM for different time periods, and then stained with acridine orange and examined under a fluorescence microscope. D, Western blot analysis of the accumulation of LC 3‐II in U87‐ MG cells that were treated with TAM (5 μmol/L) for different time period. E, p‐Akt, Akt, p‐ mTOR and mTOR protein levels were estimated by western blot analysis. F, RT ‐ <t>PCR</t> examination of autophagy pathway in mRNA expression. Beclin‐1, ATG ‐5, ATG ‐12, P62 (n = 5, * P < 0.05, ** P < .01 vs control). mTOR , mammalian target of rapamycin
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    Image Search Results


    Tamoxifen ( TAM ) induced glioma cell autophagy. A, U87‐ MG cells were treated with 5 μmol/L TAM for different time periods. Mitochondrial membrane potentia was analyzed using flow cytometry. B, Western blot analysis of the expression of P53 and Bcl‐2. C, Cells were treated with 5 μmol/L TAM for different time periods, and then stained with acridine orange and examined under a fluorescence microscope. D, Western blot analysis of the accumulation of LC 3‐II in U87‐ MG cells that were treated with TAM (5 μmol/L) for different time period. E, p‐Akt, Akt, p‐ mTOR and mTOR protein levels were estimated by western blot analysis. F, RT ‐ PCR examination of autophagy pathway in mRNA expression. Beclin‐1, ATG ‐5, ATG ‐12, P62 (n = 5, * P < 0.05, ** P < .01 vs control). mTOR , mammalian target of rapamycin

    Journal: Cancer Science

    Article Title: Estrogen receptor variant ER ‐α36 promotes tamoxifen agonist activity in glioblastoma cells

    doi: 10.1111/cas.13868

    Figure Lengend Snippet: Tamoxifen ( TAM ) induced glioma cell autophagy. A, U87‐ MG cells were treated with 5 μmol/L TAM for different time periods. Mitochondrial membrane potentia was analyzed using flow cytometry. B, Western blot analysis of the expression of P53 and Bcl‐2. C, Cells were treated with 5 μmol/L TAM for different time periods, and then stained with acridine orange and examined under a fluorescence microscope. D, Western blot analysis of the accumulation of LC 3‐II in U87‐ MG cells that were treated with TAM (5 μmol/L) for different time period. E, p‐Akt, Akt, p‐ mTOR and mTOR protein levels were estimated by western blot analysis. F, RT ‐ PCR examination of autophagy pathway in mRNA expression. Beclin‐1, ATG ‐5, ATG ‐12, P62 (n = 5, * P < 0.05, ** P < .01 vs control). mTOR , mammalian target of rapamycin

    Article Snippet: Total RNA (1 μg) was reversely transcribed using the ProtoScript II RT‐PCR kit (Takara, Dalian, China).

    Techniques: Flow Cytometry, Western Blot, Expressing, Staining, Fluorescence, Microscopy, Reverse Transcription Polymerase Chain Reaction

    Overexpression of ER ‐α36 in U251 cells leads to decreased sensitivity to tamoxifen ( TAM ) and enhanced autophagy. U251 cells overexpressed ER ‐α36. A, RT ‐ PCR examination of ER ‐α36 expression. B, MTT analysis of cell viability of glioma cells. C, Western blot analysis of the accumulation of LC 3‐II (n = 4, ** P < .01, * P < .05 vs control). ER , estrogen receptor

    Journal: Cancer Science

    Article Title: Estrogen receptor variant ER ‐α36 promotes tamoxifen agonist activity in glioblastoma cells

    doi: 10.1111/cas.13868

    Figure Lengend Snippet: Overexpression of ER ‐α36 in U251 cells leads to decreased sensitivity to tamoxifen ( TAM ) and enhanced autophagy. U251 cells overexpressed ER ‐α36. A, RT ‐ PCR examination of ER ‐α36 expression. B, MTT analysis of cell viability of glioma cells. C, Western blot analysis of the accumulation of LC 3‐II (n = 4, ** P < .01, * P < .05 vs control). ER , estrogen receptor

    Article Snippet: Total RNA (1 μg) was reversely transcribed using the ProtoScript II RT‐PCR kit (Takara, Dalian, China).

    Techniques: Over Expression, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot

    U251/ TAM shows increased levels of ER ‐α36, together with increased cell autophagy. A, RT‐PCR examination of ER‐α36 expression in U251 and U251/TAM. B, Knockdown of ER‐α36 by shRNA method. RT‐PCR examination of ER‐α36 expression in U251/TAM‐V and U251/TAM‐siRNA. C, MTT analysis of cell viability of glioma cells. D, Western blot analysis of accumulated LC 3‐II (n = 4, ** P < .01 vs control). ER , estrogen receptor; TAM, tamoxifen

    Journal: Cancer Science

    Article Title: Estrogen receptor variant ER ‐α36 promotes tamoxifen agonist activity in glioblastoma cells

    doi: 10.1111/cas.13868

    Figure Lengend Snippet: U251/ TAM shows increased levels of ER ‐α36, together with increased cell autophagy. A, RT‐PCR examination of ER‐α36 expression in U251 and U251/TAM. B, Knockdown of ER‐α36 by shRNA method. RT‐PCR examination of ER‐α36 expression in U251/TAM‐V and U251/TAM‐siRNA. C, MTT analysis of cell viability of glioma cells. D, Western blot analysis of accumulated LC 3‐II (n = 4, ** P < .01 vs control). ER , estrogen receptor; TAM, tamoxifen

    Article Snippet: Total RNA (1 μg) was reversely transcribed using the ProtoScript II RT‐PCR kit (Takara, Dalian, China).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, shRNA, Western Blot